Induksi Kalus Padi (Oryza sativa) Varietas Paketih Dengan Penambahan ZPT IAA (Indole-3-acetic acid) Dan BAP (Benzil Aminopurin) Secara In Vitro

Prayoga, Denang Dwi (2025) Induksi Kalus Padi (Oryza sativa) Varietas Paketih Dengan Penambahan ZPT IAA (Indole-3-acetic acid) Dan BAP (Benzil Aminopurin) Secara In Vitro. Undergraduate thesis, Politeknik Negeri Jember.

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Abstract

Rice (Oryza sativa L.) is a major food commodity in Indonesia that can be developed through plant tissue culture approaches. One of the techniques used is in vitro culture through callus induction as an initial step in plant propagation and plant material development. The purpose of this study was to analyze the optimal concentrations of IAA and BAP for callus induction in Paketih rice varieties. This study was conducted at the Tissue Culture Laboratory of the Jember State Polytechnic. The study was designed using the RALF method consisting of two factors. The first factor was the IAA concentration, which consisted of 0.1 mg/l, 0.2 mg/l, and 0.3 mg/l, while the second factor was the BAP concentration, which consisted of 1 mg/l, 2 mg/l, and 3 mg/l. The results showed that there was an interaction between the treatment of compact callus texture and the dominant brownish-white callus color. The 3 mg/l BAP treatment had a very significant effect on callus weight (0.95 grams). The 0.1 mg/l IAA treatment affected callus weight (0.87 grams). The interaction between IAA and BAP did not have a significant effect on callus diameter in the Paketih rice variety.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionContributorsNIDN/NIDK
Thesis advisorWardana, RudiNIDN0019028907
Uncontrolled Keywords: BAP, IAA, Induksi Kalus, Kultur Padi.
Subjects: 140 - Rumpun Ilmu Tanaman > 160 - Teknologi dalam Ilmu Tanaman > 161 - Teknologi Industri Pertanian (dan Agroteknologi)
140 - Rumpun Ilmu Tanaman > 160 - Teknologi dalam Ilmu Tanaman > 163 - Teknologi Pertanian
140 - Rumpun Ilmu Tanaman > 160 - Teknologi dalam Ilmu Tanaman > 168 - Bioteknologi Pertanian dan Perkebunan
140 - Rumpun Ilmu Tanaman > 160 - Teknologi dalam Ilmu Tanaman > 169 - Ilmu Pangan
Divisions: Jurusan Produksi Pertanian > Prodi D4 Teknik Produksi Tanaman Pangan > Tugas Akhir
Depositing User: Denang Dwi Prayoga
Date Deposited: 19 Jun 2026 00:19
Last Modified: 19 Jun 2026 00:22
URI: https://sipora.polije.ac.id/id/eprint/56528

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