Adila, Putri (2025) Optimasi Ekstraksi dan Karakterisasi Ekstrak Limbah Batang Tembakau (Nicotiana tabacum L.). Undergraduate thesis, Politeknik Negeri Jember.
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Abstract
Tobacco is an industrial crop whose leaves are mainly used as raw material for cigarette production, resulting in tobacco by-products such as stems, stalks, and roots being discarded as waste and not yet utilized to their full potential. Tobacco stalk waste, which still contains secondary metabolites, can be utilized for tobacco product diversification. Tobacco stem waste that still contains secondary metabolites can be extracted for tobacco product diversification. The extraction method used in this study was maceration. This study aimed to determine the optimal extraction conditions using ethanol solvent on the flavonoid content, phenolic content, antioxidant activity, and antimicrobial activity of the resulting extract. The factors in this study were the solvent ratio of 1:2 as the lower limit and 1:4 as the upper limit, as well as the heating temperature of 45ºC as the lower limit and 65ºC as the upper limit. The responses observed in the optimization process were the total flavonoid content, total phenolic content, and antioxidant activity. Based on the research conducted, the tobacco stem extract has a total flavonoid content of 3,58433 mg QE/ml; total phenolic content of 32,473 mg GEA/ml; and antioxidant activity with an inhibition percentage of 78,5817 and an IC50 of 36,77 ppm. The tobacco stem extract produced can also inhibit the growth of pathogenic microorganisms such as Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Listeria monocytogenes, and the fungus Aspergillus niger.
| Item Type: | Thesis (Undergraduate) | ||||||
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| Uncontrolled Keywords: | Tobacco stem, Flavonoids, Phenolics, Antioxidants, Antimicrobials | ||||||
| Subjects: | 100 - Rumpun Matematika dan Ilmu Pengetahuan Alam (MIPA) > 110 - Ilmu IPA > 112 - Kimia 140 - Rumpun Ilmu Tanaman > 160 - Teknologi dalam Ilmu Tanaman > 162 - Teknologi Hasil Pertanian 140 - Rumpun Ilmu Tanaman > 160 - Teknologi dalam Ilmu Tanaman > 163 - Teknologi Pertanian 140 - Rumpun Ilmu Tanaman > 160 - Teknologi dalam Ilmu Tanaman > 166 - Teknologi Pasca Panen |
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| Divisions: | Jurusan Teknologi Pertanian > Prodi D4 Teknologi Rekayasa Pangan > Tugas Akhir | ||||||
| Depositing User: | Putri Adila | ||||||
| Date Deposited: | 20 Aug 2025 08:17 | ||||||
| Last Modified: | 20 Aug 2025 08:17 | ||||||
| URI: | https://sipora.polije.ac.id/id/eprint/46330 |
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