Pengaruh Beberapa Macam Bahan Sterilan Eksplan Daun Pada Kultur In Vitro Kopi Arabika (Coffea Arabica L.)

Riski, Resi Nur (2021) Pengaruh Beberapa Macam Bahan Sterilan Eksplan Daun Pada Kultur In Vitro Kopi Arabika (Coffea Arabica L.). Undergraduate thesis, Politeknik Negeri Jember.

	Text (Abstrak) ABSTRACT.pdf - Accepted Version Available under License Creative Commons Attribution Share Alike. Download (122kB)
	Text (Bab 1 Pendahuluan) BAB 1 PENDAHULUAN.pdf - Submitted Version Available under License Creative Commons Attribution Share Alike. Download (3MB)
	Text (Daftar Pustaka) DAFTAR PUSTAKA.pdf - Submitted Version Available under License Creative Commons Attribution Share Alike. Download (3MB)
	Text (Laporan Lengkap) A43171226_LAPORAN LENGKAP.pdf - Submitted Version Restricted to Registered users only Download (3MB) | Request a copy

Abstract

This study aims to determine the effective sterilant in the sterilization of Arabica coffee (Coffea Arabica L.) explants. This research was carried out at the Jember State Polytechnic Network Culture Laboratory in July – August 2021. This study used a non-factorial completely randomized design (CRD) with 4 treatments and 5 replications. The treatments used were treatment A using dithane fungicide and bactericide agrimicyn, 1% (1 hour), 50% alcohol (3 seconds), NaOCl (5.25%) 0.25% (15 minutes), NaOCl (5, 25%) 0.35% (15 minutes). Treatment B used liquid detergent, dithane 2 g/L (15 minutes), 70% alcohol (3 seconds), 10% commercial bleach (7 minutes). Treatment C using detergent 20 gr/L (15 minutes), agrimycin 20 gr/L (15 minutes), erythromycin 4 gr/L (20 minutes), 70% alcohol (2 minutes), HgCL2 0.2 grams (1 minute) , HgCL2 0.2 grams (2 minutes) and treatment D used detergent, detergent 2 g/L, commercial bleach 10% (10 minutes), commercial bleach 20% (10 minutes), HgCL2 0.1% (5 minutes). Parameters observed were browning explants, fungal contamination, bacterial contamination, when fungi appeared, when bacteria appeared and live explants. Data were analyzed using variance (ANNOVA). If there are results that have a significant effect, further DMRT testing is carried out at the 5% level. The results showed that the sterilization of Arabica coffee explants in treatment D had a browning explant percentage rate of 4%, contamination percentage 4%, live explant percentage 92%, and had an average fungal appearance of 20.6 days after planting. Keywords: Sterilization Technique, Arabica Coffee, In Vitro Culture

Actions (login required)

View Item