Teknologi Delignifikasi dan Produksi Bioetanol Generasi Kedua dari Limbah Agroindustri Ampas Kelapa (Cocos Nucifer L), Ampas Tebu (Saccharum Officinarum L

Wulandari, Nuning (2019) Teknologi Delignifikasi dan Produksi Bioetanol Generasi Kedua dari Limbah Agroindustri Ampas Kelapa (Cocos Nucifer L), Ampas Tebu (Saccharum Officinarum L. Undergraduate thesis, Politeknik Negeri Jember.

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Abstract

Bioethanol is produced from the fermentation of reducing sugars as a differentiator with ethanol produced by synthesis. Bioethanol is produced from raw materials for cellulose, hemicellulose biomass and bound by lignin. Coconut pulp and sugarcane bagasse contain cellulose, hemicellulose and lignin so pre-treatment delignification is necessary. Delignification technology used is thermal and chemical delignification (NaOH and H2O2). Thermal delignification is carried out using aquadest with a mass ratio of material: aquadest is 1: 8 (w / v) and is heated for 15 minutes at 121oC in the autoclave. Delignification of NaOH is done by immersing the raw material in a 4% NaOH solution with a mass: solution ratio of 1:10 (w / v) for 12 hours. Delignification of H2O2 is done by entering 5% H2O2 with mass ratio: a solution of 1:10 (w / v) then added 2 M NaOH to pH 11.5 and heated to 50oC for 2 hours. The delignified raw material will be tested for its lignocellulose content used the Chesson Datta method. The best delignification technology results will be used at a later stage. The stages of hydrolysis used H2SO4 concentrations of 2 M and 2.5 M. Fermentation stages used 1 gram bread yeast starter with a 200 ml volume substrate. The most effective delignification method is to use 4% NaOH compared to the thermal and chemical delignification method (H2O2). Delignification of NaOH 4% can reduce levels of lignin coconut pulp up to 33.88% and sugarcane bagasse up to 25.78%. The highest bioethanol content in coconut pulp is by 2M hydrolysis of 6,11 ± 0,05 ml/L and the highest bioethanol content of sugarcane bagasse is by 2 M hydrolysis of 5,01 ± 0,05 ml/L. The highest bioethanol produced during bread yeast fermentation with coconut pulp and bagasse substrate was at fourth day.

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