Optimasi Konsentrasi NH4NO3 Dan Sukrosa Pada Media Cair Terhadap Pembentukan Umbi Mikro Kentang Secara In Vitro Dibimbing oleh Ir. Djenal, MP

Wati, Dewi Widhia (2019) Optimasi Konsentrasi NH4NO3 Dan Sukrosa Pada Media Cair Terhadap Pembentukan Umbi Mikro Kentang Secara In Vitro Dibimbing oleh Ir. Djenal, MP. Undergraduate thesis, Politeknik Negeri Jember.

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Abstract

The aim of this study was to obtain optimal concentrations of NH4NO3 and sucrose to produce large micro tubers with faster tuber formation times and high wet tuber weight. This research conducted for 4 months from January to April 2019 at the Plant Tissue Culture Laboratory, Politeknik Negeri Jember. This study used a factorial completely randomized design with two factors 9 treatments and 3 replications, 3 levels of N factors, 825 mg/l, 1650 mg/l and 2475 mg/l, 3 levels of factor S, 60 grams/l, 75 gram/l and 90 gram/l with the combination treatment of N1S1, N1S2, N1S3, N2S1, N2S2, N2S3, N3S1, N3S2, N3S3. Data were analyzed using ANOVA and then tested using a 5% DMRT level. Based on the results of the study showed the best NH4NO3 concentration for tuber current parameters and the number of tubers is 825 mg/l, and the best NH4NO3 concentration of 1650 mg/l for plant height parameters, tuber diameter and tuber weights. For the best sucrose concentration is 60 grams/l in the number of shoot parameters and the number of books, and the best sucrose concentration for the present micro tubers is 75 grams/l. The best interaction of nitrogen and sucrose for the present parameters of micro tubers is the treatment of 825 mg / l (NH4NO3) and 75 grams/l of sucrose.

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