Analisis Sistem Continue Dark Fermentation Pada Produksi Biohidrogen Limbah Kulit Pisang Raja Menggunakan Variasi Katalis Dan Konsentrasi Katalis

Soleha, Lukita Karimatus (2026) Analisis Sistem Continue Dark Fermentation Pada Produksi Biohidrogen Limbah Kulit Pisang Raja Menggunakan Variasi Katalis Dan Konsentrasi Katalis. Undergraduate thesis, Politeknik Negeri Jember.

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Abstract

Reliance on fossil fuels has driven the development of environmentally friendly renewable energy sources, one of which is biohydrogen. This study aims to analyze the effect of variations in Organic Loading Rate (OLR) and hydrogen peroxide (H₂O₂) catalyst concentration on biohydrogen production volume and Volatile Solid (VS) values, as well as to determine the optimal process conditions in a continuous dark fermentation system using plantain peel waste as a substrate. The study was conducted experimentally using OLR variations of 70, 80, and 90 g/day and H₂O₂ concentrations of 0.6, 0.8, and 1 mM. Gas volume was measured using the water displacement method, while VS was analyzed gravimetrically. Data were processed using the Response Surface Method (RSM) via Minitab 21 software. The results showed that variations in OLR and H₂O₂ concentration affected biohydrogen volume and VS values. The highest gas production observed was at an OLR of 90 g/day and an H₂O₂ concentration of 0.8 mM, yielding a volume of 28 mL, while the highest VS value was obtained at an OLR of 90 g/day and an H₂O₂ concentration of 1 mM, amounting to 93.44%. The RSM optimization results indicate that the optimal biohydrogen volume was achieved at an OLR of 90 g/day and an H₂O₂ concentration of 0.8586 mM, with a predicted volume of 26.0431 mL and a desirability of 0.9247. The optimal VS conditions were obtained at an OLR of 90 g/day and an H₂O₂ concentration of 0.8665 mM, with a VS value of 93.68% and a desirability of 1.000. The results of the study indicate that increasing the OLR and adding H₂O₂ at the optimal concentration can improve biohydrogen production performance in a continuous dark fermentation system.

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