Pengaruh Umur Planlet dan Volume Media Cair Pada Pembentukan Umbi Mikro Tanaman Kentang (Solanum Tuberosum. L) Secara In Vitro

Alif, Aprilya Faradina (2019) Pengaruh Umur Planlet dan Volume Media Cair Pada Pembentukan Umbi Mikro Tanaman Kentang (Solanum Tuberosum. L) Secara In Vitro. Undergraduate thesis, Politeknik Negeri Jember.

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Abstract

Potatoes (Solanum tuberosum L.) one source of carbohydrates for industrial raw materials, feed and biopharmaca which has the potential to be developed in Indonesia. But now the production of potatoes is decreasing. One of the main impediments to Indonesia's potato production is the lack of quality potato seeds such as potato seeds, potato tuber seeds, tuber seeds produced in vitro at reasonable prices. Micro tuber are the Product of in vitro plantlets it has a relatively small size so that handling is easier during the distribution and storage process. This research was conducted using different plantlet age and volume of liquid media with the aim to find out the exact age and volume for the formation of micro tubers. This research was conducted in January to March 2019 at the State Polytechnic in vitro Culture Laboratory in Jember. The experimental design was arranged using a factorial Randomized Completion Design (RAL), each treatment repeated 3 times. The first factor is the age of plantlets with differences in age of 10 days, 20 days and 30 days. The second factor is the volume of liquid media consisting of 20 ml, 30 ml and 40 ml in each bottle. The results of this study indicate that T2V2 (20 days plantlet age and 30 ml liquid media volume) is the best combination for tuber current parameters and micro tuber weight, 20 days plantlet age is the best age for tuber diameter parameters and 30 ml liquid media volume is the best volume for the current parameters and tuber weights.

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