KEMAMPUAN PECAH TUNAS DAN BERBIAK MOTHER PLANT PISANG RAJABULU (AAB) DAN PISANG TANDUK (AAB) DALAM MEDIUM INISIASI IN VITRO

Kasutjianingati, Kasutjianingati and Poerwanto, Roedhy and Khumaida, Nurul and Widodo, Widodo (2010) KEMAMPUAN PECAH TUNAS DAN BERBIAK MOTHER PLANT PISANG RAJABULU (AAB) DAN PISANG TANDUK (AAB) DALAM MEDIUM INISIASI IN VITRO. Jurnal Ilmiah Agriplus, 20 (1). pp. 9-17. ISSN 0854-0128

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Abstract

This research was conducted to observe the effects of different concentrations of plant growth regulators (PGRs) towards shoot multiplication on Rajabulu and Tanduk Mother Plants with AAB genome. The experiment was designed with Completely Randomized Design. The basal medium used in this experiment was MS (Murashige and Skoog, 1962), supplemented with 30 gr/l sucrose. The factors being studied are two different composition level of medium MS. The first is the medium of MA3 :MS +BA(2 mg/l)+IAA (3 mg/l) and the other is the medium of MA4: MS +BA (2 mg/l)+TDZ (0.4 μM)+IAA (3 mg/l). The results of this experiment showed that the capacity of explants to form shoots depend upon cultivars. Addition of TDZ and IAA into the media had significant effects on the number of shoots/cormlet. The cultivar of Rajabulu (AAB) showed a low rate of shoot multiplication, whilst Tanduk (AAB) showed a high rate of shoot/cormlet multiplication and tended to form noduls. This lead to a conclusion that MA4 is significant to stimulate the shoot break for both cultivars. The best result for Rajabulu is up to subculture 2-3 by 2.50-3.50 shoots, whilst for Tanduk up to subculture 1-2 by 2.83-9.67 shoot.

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