PENGARUH BAKTERI ENDOFIT TERHADAP MULTIPLIKASI TUNAS RAJABULU SECARA IN VITRO

Kasutjianingati, Kasutjianingati and Poerwanto, Roedhy and Widodo, Widodo and Khumaida, Nurul (2010) PENGARUH BAKTERI ENDOFIT TERHADAP MULTIPLIKASI TUNAS RAJABULU SECARA IN VITRO. Jurnal Ilmiah Agriplus, 20 (3). pp. 227-232. ISSN 0854-0128

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Abstract

This research was conducted toobserve the effects of endophytic bacteria on Rajabulu (AAB), by observing the growth and the quality of shoot formed. The experiment was designed with Completely Randomized Design, the experiment have 3 factors, the first factor is two kinds of rhyzobateria which is P. fluorescens -ES32, B. substilis -SB3 and without rhyzobateria; the second factor is 8 kinds of media, i.e. MS0 (without PGR and withoutTSB ), MS0 + 10% TSB, MS0 + 20% TSB, MS0 + 30% TSB, MS + PGR (BA 2 mg l-1 + IAA 0,5 mg l-1), MS + PGR + 10% TSB, MS + PGR + 20% TSB, MS + PGR + 30% TSB; the three factor is two kinds of aplication bacteria (the explant were immersed in the bacterial suspession;hurted by needle which were immersed in the bacterial suspession). The experiment results were the use of endophytic bacteria (P. fluorescens -ES32 or B. substilis –SB3) in media MS0 (without PGR) produced more shoots (0.63-2.17shoots/explan) compared to media MS0 (without PGR; without the bacteria = 017shoots/explan)

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