Pengaruh Beberapa Komposisi Media Alternatif Sebagai Media Pertumbuhan Cendawan Metarhizium anisopliae

Hasanah, Anisa Aprilia (2023) Pengaruh Beberapa Komposisi Media Alternatif Sebagai Media Pertumbuhan Cendawan Metarhizium anisopliae. Diploma thesis, Politeknik Negeri Jember.

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Abstract

M. anisopliae is one of the entomopathogenic fungi and antagonistic fungi available in the world and acts as a biological agent because it has the ability to control Plant Disturbing Organisms (OPT) ranging from the order Coleoptera, Hemiptera, Orthoptera, Hymenoptera, Isoptera and Lepidoptera. Currently, the use of entomopathogenic fungi has problems in providing ready-to-use isolates. The provision of these ready-to-use isolates depends on the propagation technique and also the medium used against the abundance of M. anisopliae fungi in each treatment. This study aims to determine and develop the fungus M. anisopliae as a biological agent with the best media according to the concentration composition. This research was carried out in June – December 2023 at the Jember State Polytechnic Plant Protection Laboratory using a factorial Complete Randomized Design (RAL) with 5 levels of treatment and 3 repeats on 3 different media so that 45 experimental units were obtained, namely Corn Rice Media (M1), Corn Rice Media + Fine Rice Bran (M2), Corn Rice + Coarse Rice Bran (M3) with a combination of 5grams / 100 ml concentration, 10grams/100 ml, 15grams/100 ml, 20grams/100 ml, 25grams/100ml. The results of the study after 14 days of incubation period showed the results that the highest average spore density was in the treatment of corn rice mixture media + coarse rice bran with a ratio of 1: 1 solution concentration of 25grams media / 100 ml aquadest. Media mixture of corn rice + coarse rice bran is the best treatment that contains complete nutrients from the adoption of proteins, fats and carbohydrates. Keywords: entomopathogenic fungus, exploration, M. anisopliae

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